Detection of hepatitis B virus core antigen by phage display mediated TaqMan real-time immuno-PCR
نویسندگان
چکیده
منابع مشابه
Phage display mediated immuno-PCR
Immuno-PCR (IPCR) is a powerful detection technology in immunological study and clinical diagnosis due to its ultrasensitivity. Here we introduce a new strategy termed phage display mediated immuno-PCR (PD-IPCR). Instead of utilization of monoclonal antibody (mAb) and chemically bond DNA that required in the conventional IPCR, a recombinant phage particle is applied as a ready reagent for IPCR ...
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Quantitation of hepatitis B virus (HBV) DNA in serum is a useful method for the monitoring of HBV replication. We attempted to develop a quantitative assay system for HBV DNA that is more sensitive, accurate, and reproducible than existing systems. We detected HBV DNA by real-time detection PCR (RTD-PCR) based on Taq Man chemistry. The efficacy of this assay was evaluated by quantitatively meas...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2013
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2012.09.017